THE SYNTHESIS OF IMMUNOGLOBULIN CONJUGATES WITH DEHYDROGENASES
V. L. MARKINA, E. I. KARASYOVA, A. N. ERYOMIN
Institute of Bioorganic Chemistry, Academy of Sciences of the Byelorussian SSR, Minsk
Abstract: Three methods of synthesis of immunoglobulin conjugates with malate, lactate and glucose-6-phosphate dehydrogenases, involving the sodium metaperiodate oxidation of immunoglobulin carbohydrate component, use of water-soluble carbodiimide and the one-step glutaraldehyde technique, were compared. The glutaraldehyde method was shown to give immunoglobulin-dehydrogenase conjugates with high catalytic and im-munochemical activity, which may be useful for enzyme-immunoassay.
Russian Journal of Bioorganic Chemistry 1990, 16 (3):318-323