THE KINETIC CHARACTERISTICS OF THE NEUROLEPTIC BINDING SITES ON HUMAN LYMPHOCYTES

I. I. IZUMRUDOVA, S. V. ZAITSEV, A. A. KOSHKIN, N. V. PORODENCO*, S. D. VARFOLOMEEV

M. V. Lomonosov Moscow State University; *Institute of Applied Molecular Biology, Ministry of Health of the USSR, Moscow

Abstract: The kinetics and equilibrium of the interaction of [3H]spiperone with binding sites on human lymphocytes have been studied using radioligand analyses. The process of [3H]spiperone binding to these sites can be explained by the model of ligand interaction with two independent binding sites (high-affinity site, K_D1=3 nM, and low-affinity site, K_D2—2Q nM), thus confirming the heterogeneity of the sites on human lymphocytes.

Russian Journal of Bioorganic Chemistry 1990, 16 (2):272-274

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