STUDIES ON THE RECEPTOR FOR NEUROTOXIN FROM BLACK WIDOW SPIDER VENOM. II. ISOLATION AND PROPERTIES OF THE RECEPTOR FROM BOVINE BRAIN MEMBRANE
A. G. PETRENKO, I. N. SURKOVA, O. G. SHAMOTIENKO, V. A. KOVALENKO, V. N. KRASNOPEROV, L. A. TRETJYAKOV*, Yu. A. USHKARYOV, E. V. GRISHIN
M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow; * V. I. Palladin Institute of Biochemistry, Academy of Sciences of the Ukrainian SSR, Kiev
Abstract: Biospecific sorbents for isolation of the latrotoxin receptor have been obtained and studied. Binding of the receptor components solubilized from the bovine cerebral cortex membrane to the immobilized toxin critically depends on the presence of calcium ions and the solution ionic strength. The procedure for semipreparative isolation of the highly active receptor preparation with Kd=9·10-10°M and Bmax=0.9 nmol/mg has been developed. Binding activity of the isolated receptor is inhibited by heating as well as by proteases and denaturing agents. According to electrophoretic analysis in the presence of SDS the receptor complex contains protein components of molecular mass 200, 160, 79, 43 kDa, the former two being glycoproteins.
Russian Journal of Bioorganic Chemistry 1990, 16 (2):158-165