Identification of interaction sites of tRNALeu from cow mammary gland with the cognate aminoacyl-tRNA synthetase by the chemical modification method

Z. M. Petrushenko, M. A. Tukalo, O. I. Gudzera, O. T. Rozchko, G. Kh. Matsuka

Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR, Kiev

Abstract: The interaction of the cow mammary gland tRNALeuIAG, having a long variable loop, with the cognate aminoacyl-tRNA synthetase has been studied by the alkylation with ethylnitrosourea. It was shown that leucyl-tRNA synthetase protects from alkylation 3'-phosphates of the nucleotides 1213 in D-loop, 2324 in D-stem and 3743 in the anticodon arm of tRNALeuIAG. All regions of interaction with the aminoacyl-tRNA synthetase are located in the same plane of tRNA whereas the long variable loop is in another plane.

Russian Journal of Bioorganic Chemistry 1990, 16 (12):1647-1652

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