An efficient variant of the solid phase H-phosphonate synthesis of oligodeoxyribonucleotides

I Ya. Dubey, T. V. Lyapina, D. M. Fedoryak

Institute of Bioorganic and Oil Chemistry, Academy of Sciences of the Ukrainian SSR, Kiev

Abstract: An efficient variant of the H-phosphonate method of the oligonucleotido synthesis is described. It is based on the use of acetonitrile quinoline (4:1) mixture as the condensation reaction medium, where the rate of the bis-acylphosphite formation is significantly lower than in pyridine mixtures, thus eliminating the negative effect of preacti-vation of the P-component on the synthesis efficiency. Pentadecamer (Tp)14T was obtained in a high yield at the Victoria-4M automatic synthesizer using a Silochrom S-80 based polymer support.

Russian Journal of Bioorganic Chemistry 1990, 16 (11):1574-1576

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