A method for the splitting of the carbohydrate chains from N-glycoproteins with NaBH4–BaCl2 or SrCl2
L. M. Likhosherstov, O. S. Novikova, V. A. Derevitskaya, N. K. Kochetkov
N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow
Abstract: A reductive system NaBH4–BaCl2 or SrCl2 is proposed to split the carbohydrate chains of N-glycoproteins. The method allows one to obtain intact oligosaccharides (in 50–60% yields) including alkali-labile fucosylated oligosaccharides from plant N-glycoproteins (lectin from Sambucus nigra bark).
Russian Journal of Bioorganic Chemistry 1990, 16 (10):1386-1392