A method for the splitting of the carbohydrate chains from N-glycoproteins with NaBH₄–BaCl₂ or SrCl₂

L. M. Likhosherstov, O. S. Novikova, V. A. Derevitskaya, N. K. Kochetkov

N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow

Abstract: A reductive system NaBH₄–BaCl₂ or SrCl₂ is proposed to split the carbohydrate chains of N-glycoproteins. The method allows one to obtain intact oligosaccharides (in 50–60% yields) including alkali-labile fucosylated oligosaccharides from plant N-glycoproteins (lectin from Sambucus nigra bark).

Russian Journal of Bioorganic Chemistry 1990, 16 (10):1386-1392

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