Formylphenyl esters of guanosine-5'-mono-, di- and triphosphates at the terminal phosphate residue

M. A. Grachev, E. A. Lukhtanov, A. A. Mustaev

Limnological Institute, Siberian Division, Academy of Sciences of the USSR, Irkutsk

Abstract: Nine nucleotide formylphenyl esters at the terminal phosphate residue have been obtained via N-methylimidazolides (for GMP and GDP) or trimetaphosphate (lor GTP) by the reaction with a formylphenol (4-hydroxy-, 4-hydroxy-3-methoxy-, or 2-hydroxyben-zaldehyde) and characterized by UV spectroscopy, ion-exchange chromatography, and reversed-phase HPLC. They are stable towards phosphomonoesterase. Some of them have been used for highly selective affinity labelling of the E. coli RNA polymerase.

Russian Journal of Bioorganic Chemistry 1990, 16 (10):1379-1385

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