The RNA cleavage by hybridase. II. Automatic synthesis of oligo(ribodeoxyribo)nucleotide probes

E. A. Romanova, T. S. Oretskaya, V. V. Sukhomlinov, N. F. Krynetskaya. V. G. Metelev, Z. A. Shabarova

Department of Chemistry, M. V. Lomonosov Moscow State University

Abstract: Several oligo(ribodeoxyribo)nucleotides to be used as probes in the RNase H-induced hydrolyses of 5S ribosomal RNA E. coli were synthesized on the Victoria-4M automatic gene synthesizer by the phosphoramidite approach, which allows for introducing ribonuc-leotides into any position of the oligomer. 2'-Hydroxy function was protected by tert-butyldimethylsilyl group whose hydrophobicity simplified isolation of the oligonucleotides-by reverse-phased HPLC.

Russian Journal of Bioorganic Chemistry 1990, 16 (10):1348-1354

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