PURIFICATION AND CHARACTERIZATION OF A THERMALLY STABLE YELLOW LACCASE FROM DAEDALEA FLAVIDA MTCC-145 WITH HIGHER CATALYTIC PERFORMANCE TOWARDS SELECTIVE SYNTHESIS OF SUBSTITUTED BENZALDEHYDES

2016 M. Sharma, P. K. Chaurasia#, A. Yadav, R. S. S. Yadav, S. Yadava, and K. D. S. Yadav

#E-mail: pankaj.chaurasia31@gmail.com

Department of Chemistry, D.D.U. Gorakhpur University, Uttar-Pradesh, 273009 India

Received June 6, 2015; in final form, August 24, 2015

A laccase from the culture filtrate of white rot fungus Daedalea flavida MTCC-145 has been purified and characterized. The method involved concentration of the culture filtrate by ultrafiltration and an anion exchange chromatography on diethylaminoethyl (DEAE) cellulose. The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and native polyacrylamide gel electrophoresis (native PAGE) both gave single protein bands indicating that the enzyme preparation was pure. The molecular mass of the enzyme determined from SDS-PAGE analysis was 75.0 kDa. Purification fold was 21.5 while recovery of the enzyme activity was 11.52%. Using 2,6-dimethoxyphenol, diammonium salt of 2,2'-[azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)] and 3,5-dimethoxy-4-hydroxybenzaldehyde azine as substrates, the Km, kcat, and kcat/Km values of the laccase were found to be 440 μM, 6.45 s1, 1.47 x 104 M1 s1; 366 μM, 6.45 s1, 1.76 x 104 M1 s1; and 226 μM, 6.45 s1, 2.85 x 104 M1 s1, respectively. The pH and temperature optima were 4.5 and 50C, respectively. The enzyme was most stable at pH 5.0 when exposed for 1 h. The purified laccase has yellow color and shows no absorption band around 610 nm characteristic of blue laccases. The enzyme transforms toluene and substituted toluenes to corresponding benzaldehyde and substituted benzaldehydes in the absence of mediator molecules with higher catalytic efficiency as compared to other known laccases.

Keywords: copper containing enzymes, yellow laccases, lignolytic enzymes, Daedalea flavida, 2,6-dimethoxyphenol.

. 2016, 42 (1): 69-78