MULBERRY (Morus L.) METHIONINE SULFOXIDE RREDUCTASE GENE CLONING, SEQUENCE ANALYSIS, AND EXPRESSION IN PLANT DEVELOPMENT AND STRESS RESPONSE

Wei Tonga, 1, Yinghua Zhanga, 1, Heng Wanga, Feng Lia, Zhaoyue Liua, Yuhua Wanga, b, Rongjun Fanga, c, Weiguo Zhaoa, b, #, Long Lia, b, #

#E-mail: wgzsri@126.com; seri68@hotmail.com

aCollege of Biological and Chemical Engineering, Jiangsu University of Science and technology, Zhenjiang Jiangsu 212018, China;
bSericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang Jiangsu 212018, China;
cSchool of Life Science, Nanjing University, Nanjing Jiangsu 210093, China

Running title:Mulberry MSR gene cloning and expression

Received February 27, 2013; in final form, April 23, 2013

Methionine sulfoxide reductase plays a regulatory role in plant growth and development, especially in scavenging reactive oxygen species by restoration of the oxidation of methionine in protein. A full-length cDNA sequence encoding methionine sulfoxide reductase (MSR) from mulberry, which we designated MMSR, was cloned based on mulberry expressed sequence tags (ESTs). Sequence analysis showed that the MMSR is 810 bp long, encoding 194 amino acids with a predicted molecular weight of 21.6 kDa and an isoelectric point of 6.78. The expression level of the MMSR gene under conditions of drought and salt stresses was quantified by qRT-PCR. The results show that the expression level changed significantly under the stress conditions compared to the normal growth environment. It helps us to get a better understanding of the molecular basis for signal transduction mechanisms underlying the stress response in mulberry.

Keywords: mulberry, methionine sulfoxide reductase, gene cloning, induced expression

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1The first two authors contributed equally

Биоорг.химия 2013, 39 (5): 586-593