Polycationic Catalysts for Phosphodiester Bond Cleavage on the Basis of 1,4-Diazabicyclo[2.2.2]octane

E.†A.†Burakova1†#, N.†A.†Kovalev1, I.†L.†Kuznetzova1, M.†A.†Zenkova1, V.†V.†Vlassov1 and V.†N.†Silínikov1

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1Novosibirsk Institute of Chemical Biology and Fundamental Medicine, Siberian Division, Russian Academy of Sciences, pr. Akademika Lavrentíeva 8, Novosibirsk, 630090, Russia

Received: June†22, 2006;††in final form: July†11, 2006

Abstract.††A number of tetracationic compounds capable of phosphodiester bond cleavage within a 21-membered ribooligonucleotide were designed and synthesized. The artificial ribonucleases represent two residues of quaternized 1,4-diazabicyclo[2.2.2]octane bearing alkyl substituents of various lengths and connected with a rigid linker. The efficiency of cleavage of phosphodiester bonds in an RNA target depends on the linker structure and the length of alkyl substituent.

Key words:††artificial ribonucleases;†1,4-diazabicyclo[2.2.2]octane;†polycationic molecules

Russian Journal of Bioorganic Chemistry 2007, 33(5): 523-530