Development of a Rapid Method for the Detection of Prostate-Specific Antigen by Immunochromatography
I. A. Lubavina1 #, A. A. Zinchenko1, Yu. S. Lebedin2 and S. V. Chukanov2
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1 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia; 2 Khema-Medica Co. Ltd., Moscow, Russia
Received: July 11, 2006; in final form: September 13, 2006
Abstract. A single-step qualitative rapid test for the determination of prostate-specific antigen (PSA) in samples of human blood serum by immunochromatography using a complex of colloidal gold with monoclonal antibodies to PSA as the detection agent was developed. The determination limit for PSA in serum blood samples is 10 ng/ml; the analysis time, 15–25 min; the sensitivity of the method, 100%; and its specificity, 92.5%.
Key words: complex with colloidal gold; colloidal gold; immunochromatography; monoclonal antibodies to prostate-specific antigen; prostate-specific antigen; determination
Russian Journal of Bioorganic Chemistry 2007, 33(5): 511-515