Synthetic Analogues of Antimicrobial Peptides from the Venom of the Central Asian Spider Lachesana Tarabaevi

A. A. Vassilevski1 #, S. A. Kozlov1, M. N. Zhmak1, I. A. Kudelina1, P. V. Dubovskii1, O. Ya. Shatursky2, A. S. Arseniev1 and E. V. Grishin1

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1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, ul. Leontovicha 9, Kiev, 01601, Ukraine

Received: June  26, 2006;  in final form: October  31, 2006

Abstract.  Analogues of latarcins Ltc1 and Ltc3b, antimicrobial peptides from the venom of the Central Asian spider Lachesana tarabaevi capable of formation of amphiphilic structures in membranes without involvement of disulfide bonds, were synthesized. The amino acid sequences of the analogues correspond to immature forms of these peptides, each of them containing an additional C-terminal amino acid residue. It is concluded from the study of the biological activity of the synthesized peptides that the posttranslational C-terminal amidation of Ltc3b is a functionally important modification that ensures a high activity of the mature peptide. The lipid composition was shown to affect the interaction of synthesized peptides with artificial membranes. The analogue of Ltc3b manifested the highest activity on cholesterol-containing membranes. The mechanism of action of the studied antimicrobial peptides on membranes is discussed.

Key words:  amphiphilic helix, antimicrobial peptides, bilayer lipid membranes, liposomes, posttranslational modifications

Russian Journal of Bioorganic Chemistry 2007, 33 (4): 376-382