Structure of Carbohydrate Chains of Fucolectin from the Bark of Golden Rain Shrub Laburnum anagyroides
V. E. Piskarev1# and I. A. Yamskov1
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1Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, ul. Vavilova 28, Moscow, 119991, Russia
Received: July 18, 2006; in final form: September 4, 2006
Abstract. A reductive LiBH4-ButOH cleavage of N-glycosylamide carbohydrate-peptide bond allowed splitting off of oligosaccharide chains of the fucolectin, the bark agglutinin from the shrub golden rain Laburnum anagyroides (LABA). Four N-glycans were isolated by HPLC, and their structures were elucidated by monosaccharide analysis and 1H NMR (500 MHz) spectroscopy: Man2Fuc1Xyl1GlcNAc2 (M2FX), Man3Xyl1GlcNAc2 (M3X), Man3Fuc1Xyl1GlcNAc2 (M3FX), and Man3Xyl1Fuc1GlcNAc3 (NM3FX). All the N-glycans contain D-xylose and three of them, L-fucose; they were found to be in a 1:8:3:1 ratio.
Key words: fucolectin, 1H NMR spectroscopy, Laburnum anagyroides, N-glycan, N-glycoprotein
Russian Journal of Bioorganic Chemistry 2007, 33 (1): 61-65