Structural Studies

The Structure of the Glycerophosphate-Containing O-Specific Polysaccharide from Escherichia coli O130

A. V. Perepelov¹, B. Lu^2,3, S. N. Senchenkova¹, S. D. Shevelev¹, W. Wang^2,3, A. S. Shashkov¹, L. Feng^2,3, L. Wang^2,3 and Yu. A. Knirel^1# 

^#Phone: (495) 137-61-48; fax: (495) 137-61-48; e-mail: knirel@ioc.ac.ru

¹Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii pr. 47, Moscow, 119991, Russia;
²TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa street, TEDA, Tyan-jin, 300457, Chinese People’s Democratic Republic;
³Tyan-jin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, 23 HongDa street, TEDA, Tyan-jin, 300457, Chinese People’s Democratic Republic

Received: March 2, 2006;  in final form: March 28, 2006

Abstract. A phosphorylated O-specific polysaccharide was obtained by mild acidic degradation of the lipopolysaccharide from the enteric bacterium Escherichia coli O130 and characterized by the methods of chemical analysis, including dephosphorylation and ¹H and ¹³C NMR spectroscopy. The polysaccharide was shown to be composed of branched tetrasaccharide repeating units containing two N-acetyl-D-galactosamine residues, D-galactose, D-glucose, and glycerophosphate residues (one of each). The polysaccharide has the following structure, which is unique among the known bacterial polysaccharides:

Key words: Escherichia coli, O-specific polysaccharide, glycerophosphate, structure, NMR spectroscopy

Russian Journal of Bioorganic Chemistry 2007, 33 (1): 57-60