Isolation and Elucidation of Structure of Peptides from Oat ( Avena sativa ) Seedlings

O. G. Gara a,b , # O. N. Yatskin a , V. I. Shvets b , A. A. Karelin a , and V. T. Ivanov a

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a Shemyakin--Ovchinnikov Institute of Bioorganic Chemistry , Russian Academy of Sciences , ul. Miklukho-Maklaya 16/10 , Moscow , 117997 Russia

b Lomonosov State Academy of Fine Chemical Technology, pr. Vernadskogo 86, Moscow, 119571 Russia

Received August 18, 2005; in final form, September 2, 2005

Abstract: The level of proteolytic activity in tissues of oat seedlings was characterized under acidic conditions, and the number and content of the main components in low-molecular-mass fractions of the extract were determined. The structures of the majority of predominant peptide components isolated from the extract were studied. The use of a database of protein structures helped suggest possible structures of protein precursors of the peptides isolated. Detailed information on a plant peptidome was obtained for the first time.

Key words: peptides of plant tissues, isolation, identification, protein precursor

Russian Journal of Bioorganic Chemistry 2006, 32 (2):191-200