Short Peptide Fragments with Antiulcer Activity from a Collagen Hydrolysate

Yu. A. Zolotarev a , # K. E. Badmaeva b , Z. V. Bakaeva b , G. E. Samonina b , G. N. Kopylova b , A. K. Dadayan a , Yu. B. Zverkov a , S. K. Garanin c , B. V. Vaskovsky c , I. P. Ashmarin b , and N. F. Myasoedov a

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a Institute of Molecular Genetics, Russian Academy of Sciences, pl. akademika Kurchatova 2, Moscow, 123182 Russia

b Faculty of Biology, Moscow State University , Vorob'evy gory, Moscow , 117234 Russia

c Shemyakin--Ovchinnikov Institute of Bioorganic Chemistry , Russian Academy of Sciences , ul. Miklukho-Maklaya 16/10, Moscow 117997 Russia

Received July 4, 2005; in final form, July 6, 2005

Abstract: A peptide acidic hydrolysate of collagen (PHC) was obtained under conditions (4 N HCl) ensuring the predominant formation of short peptides, glyprolines. They were separated and their antiulcer activity was studied. Thirty individual peptides with molecular masses of 174--420 amu were isolated from the PHC by HPLC. The PHC was shown to predominantly contain 2- to 4-aa peptides, including PG, GP, and PGP. Experiments on rats demonstrated that, on intragastric administration at a dose of 1 mg/kg, PHC enhances the stability of the gastric mucosa to the action of ulcerogenic factors, such as ethanol and stress, and exhibits a protecting antiulcer effect. Even a lesser dose (0.1 mg/kg), which reduced ulcer area twofold, was effective in the stress model of ulcer formation. The intraperitoneal and intragastric administration of PHC at a dose of 1 mg/kg was found to exhibit a therapeutic effect in the acetate model of ulcer formation.

Key words: antiulcer activity, glyprolines, peptides, stomach ulcer models

Russian Journal of Bioorganic Chemistry 2006, 32 (2):174-178