Mechanisms of Peroxidase Oxidation of o-Dianisidine, 3,3',5,5'-Tetramethylbenzidine, and o-Phenylenediamine in the Presence of Sodium Dodecyl Sulfate

A. V. Kireiko,# I. A. Veselova, and T. N. Shekhovtsova

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Faculty of Chemistry, Moscow State University, Vorob'evy gory, Moscow, 119992 Russia

Peroxydase oxidation of o-dianisidine, 3,3',5,5'-tetramethylbenzidine, and o-phenylenediamine in the presence of sodium dodecyl sulfate (SDS),an anionic surfactant, was spectrophotometrically studied. It was found that 0.1-100 mM SDS concentrations stabilize intermediates formed in the peroxydase oxidation of these substrates. The cause of the stabilization is an electrostatic interaction between positively charged intermediates and negatively charged surfactant.

Key words: o- dianisidine ; horseradish iperoxydase , oxidation mechanism; o- phenylenediamine ; sodium dodecyl sulfate; 3 ,3',5,5' -tetramethylbenzidine

Received June 29, 2005; in final form, August 10, 2005

Russian Journal of Bioorganic Chemistry 2006, 32 (1):71-77